IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.
Identifieur interne : 004727 ( Main/Exploration ); précédent : 004726; suivant : 004728IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.
Auteurs : D S Hwang [États-Unis] ; B. Thöny ; A. KornbergSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN bactérien (biosynthèse), Chromosomes de bactérie, Données de séquences moléculaires, Escherichia coli (génétique), Plasmides, Protéines Escherichia coli, Protéines bactériennes (biosynthèse), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (biosynthèse), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
- MESH :
- biosynthèse : ADN bactérien, Protéines bactériennes, Protéines de liaison à l'ADN.
- génétique : Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- métabolisme : Protéines bactériennes, Protéines de liaison à l'ADN.
- Chromosomes de bactérie, Données de séquences moléculaires, Plasmides, Protéines Escherichia coli, Réplication de l'ADN, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Bacterial Proteins (biosynthesis), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Base Sequence, Chromosomes, Bacterial, DNA Replication, DNA, Bacterial (biosynthesis), DNA-Binding Proteins (biosynthesis), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Electrophoresis, Polyacrylamide Gel, Escherichia coli (genetics), Escherichia coli Proteins, Molecular Sequence Data, Plasmids.
- MESH :
- chemical , biosynthesis : Bacterial Proteins, DNA, Bacterial, DNA-Binding Proteins.
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins.
- genetics : Escherichia coli.
- Base Sequence, Chromosomes, Bacterial, DNA Replication, Electrophoresis, Polyacrylamide Gel, Escherichia coli Proteins, Molecular Sequence Data, Plasmids.
Abstract
Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S., and Kornberg, A. (1990) Cell 63, 325-331). Isolation of the iciA gene (Thöny, B., Hwang, D.S., Fradkin, L., and Kornberg, A. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4066-4070) has made possible the construction of an IciA-overproducing strain, which in turn has simplified the isolation of a large quantity of the protein, indistinguishable from that of the wild-type strain. Based on sedimentation and gel filtration, the IciA protein is an elongated dimer of a 33.4-kDa subunit. The specific binding of IciA protein to the 13-mers was stable for 2 h at 30 degrees C. The amounts of IciA protein, detected by immunoassays, increased 4-fold compared with levels (about 100 dimers) in log-phase cells whereas levels of DnaA protein decreased upon entry of cells into the stationary phase.
PubMed: 1733927
Affiliations:
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Le document en format XML
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Kornberg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1733927</idno><idno type="pmid">1733927</idno><idno type="wicri:Area/PubMed/Corpus">002967</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002967</idno><idno type="wicri:Area/PubMed/Curation">002967</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002967</idno><idno type="wicri:Area/PubMed/Checkpoint">002808</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002808</idno><idno type="wicri:Area/Ncbi/Merge">000509</idno><idno type="wicri:Area/Ncbi/Curation">000509</idno><idno type="wicri:Area/Ncbi/Checkpoint">000509</idno><idno type="wicri:doubleKey">0021-9258:1992:Hwang D:icia:protein:a</idno><idno type="wicri:Area/Main/Merge">004798</idno><idno type="wicri:Area/Main/Curation">004727</idno><idno type="wicri:Area/Main/Exploration">004727</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.</title><author><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemistry, Stanford University School of Medicine</wicri:cityArea></affiliation></author><author><name sortKey="Thony, B" sort="Thony, B" uniqKey="Thony B" first="B" last="Thöny">B. Thöny</name></author><author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="ISSN">0021-9258</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (biosynthesis)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Base Sequence</term><term>Chromosomes, Bacterial</term><term>DNA Replication</term><term>DNA, Bacterial (biosynthesis)</term><term>DNA-Binding Proteins (biosynthesis)</term><term>DNA-Binding Proteins (genetics)</term><term>DNA-Binding Proteins (metabolism)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Escherichia coli (genetics)</term><term>Escherichia coli Proteins</term><term>Molecular Sequence Data</term><term>Plasmids</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (biosynthèse)</term><term>Chromosomes de bactérie</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Plasmides</term><term>Protéines Escherichia coli</term><term>Protéines bactériennes (biosynthèse)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines de liaison à l'ADN (biosynthèse)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Réplication de l'ADN</term><term>Séquence nucléotidique</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Bacterial Proteins</term><term>DNA, Bacterial</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN bactérien</term><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines bactériennes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Chromosomes, Bacterial</term><term>DNA Replication</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Escherichia coli Proteins</term><term>Molecular Sequence Data</term><term>Plasmids</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromosomes de bactérie</term><term>Données de séquences moléculaires</term><term>Plasmides</term><term>Protéines Escherichia coli</term><term>Réplication de l'ADN</term><term>Séquence nucléotidique</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S., and Kornberg, A. (1990) Cell 63, 325-331). Isolation of the iciA gene (Thöny, B., Hwang, D.S., Fradkin, L., and Kornberg, A. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4066-4070) has made possible the construction of an IciA-overproducing strain, which in turn has simplified the isolation of a large quantity of the protein, indistinguishable from that of the wild-type strain. Based on sedimentation and gel filtration, the IciA protein is an elongated dimer of a 33.4-kDa subunit. The specific binding of IciA protein to the 13-mers was stable for 2 h at 30 degrees C. The amounts of IciA protein, detected by immunoassays, increased 4-fold compared with levels (about 100 dimers) in log-phase cells whereas levels of DnaA protein decreased upon entry of cells into the stationary phase.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name><name sortKey="Thony, B" sort="Thony, B" uniqKey="Thony B" first="B" last="Thöny">B. Thöny</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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